Fig. 1

Experimental flowchart. First, the newly resected tumors were cut along their long axis. Next, specimens were obtained from T1-5 sites on the long axis (T1 is the midpoint of the long axis of a tumor; T5 is the outer edge of the long axis of the tumor; T3 is the midpoint of the line between T1 and T5; T2 is the midpoint of the line between T1 and T3; T4 is the midpoint of the line between T3 and T5), and were divided into two groups. In one group, the specimens were placed in 10% formalin for subsequent immunohistochemistry, and in another group, the specimens were placed in liquid nitrogen for subsequent RNA-seq. Finally, the IHC and RNA-seq results were quantified, and the similarity of the TIME in different tumor regions was analyzed using the Mahalanobis distance. FFPE, formalin-fixed and paraffin-embedded; IHC, Immunohistochemistry; and RNA-seq, RNA sequencing