Fig. 2

IFIT3 promoted HNSC cell growth and cell stemness in vitro. A Verification of transfection efficiency of IFIT3 knockdown in FaDu and Cal 27 cells at mRNA and protein levels. B Verification of transfection efficiency of IFIT3 up-regulation in FaDu and Cal 27 cells at mRNA and protein levels. C The viability of IFIT3 knockdown in FaDu and Cal 27 cells was assessed via CCK-8 assay. D The viability of IFIT3 up-regulation in FaDu and Cal 27 cells was assessed via CCK-8 assay. E The impact of IFIT3 knockdown on the clone formation ability in FaDu and Cal 27 cells. F The impact of IFIT3 up-regulation on the clone formation ability in FaDu and Cal 27 cells. G Representative images of CD44⁺ and CD44⁻ cells isolated from FaDu and Cal 27 cells after 10 days in serum-free culture media. Scale bar, 200 μm. H Flow cytometry detected the distribution of CD44⁺ cells. Scale bar, 200 μm. I The sphere formation ability of IFIT3 knockdown in FaDu and Cal 27 cells. Scale bar, 200 μm. J The sphere formation ability of IFIT3 up-regulation in FaDu and Cal 27 cells. Scale bar, 200 μm