Table 1 Primary antibodies and their conditioning in this study
From: Apoptosis-related factors are relevant to progression of pancreatic neuroendocrine tumors
Antibody | Clone | Dilution | Condition | Source |
|---|---|---|---|---|
GLUT1 | Rabbit monoclonal (EPR3915) | 1/500 | pH 6.0, overnight | Abcam, Cambridge, UK |
Survivin | Rabbit polyclonal (BC008718) | 1/200 | pH 9.0, overnight | Proteintech, Rosemont, IL, USA |
XIAP | Rabbit polyclonal (BC032729) | 1/100 | pH 9.0, overnight | Proteintech, Rosemont, IL, USA |
Caspase3 | Rabbit monoclonal (E87) | 1/100 | pH 9.0, overnight | Abcam, Cambridge, UK |
SSTR2a | Rabbit monoclonal (UMB1) | 1/200 | pH 6.0, overnight | Abcam, Cambridge, UK |
SSTR5 | Rabbit monoclonal (UMB4) | 1/200 | pH 9.0, overnight | Abcam, Cambridge, UK |
Ki-67 | Mouse monoclonal (MM1) | Diluted | pH 6.0, overnight | Leica Biosystems, Newcastle, UK |