Table 1 Organoids models and their application for immune researches in BC
From: Breast cancer organoids and their applications for precision cancer immunotherapy
Authors | Years | BC cells | Co-culture cells | Approaches | Application |
|---|---|---|---|---|---|
Hanley, C. J. et al. [48] | 2020 | Primary BC cells | ECM | Adding stroma cells to organdids | Reveal dynamic molecular interactions between stromal cells and cancer cells |
Dhimolea, E. et al. [49] | 2021 | HR+ BC cells | ECM +/− BMSC | Adding cells to organdids | Characterize the pleiotropic hormone-independent mechanisms in HR+ BC tumors |
Zumwalde et al. [47] | 2016 | Human breast ductal epithelial cells | Vδ2+ T lymphocytes | Retaining and expanding endogenous immune cells | Demonstrate the ability of Vδ2(+) T cells to respond to bisphosphonate drugs as an immunotherapy in BC |
Aboulkheyr Es, H. et al. [58] | 2022 | PDOTS | Original TME and infiltrated immune cells | 3D microfluidic device | The real-time screening of conventional chemotherapy agents |
Truong, D. D. et al. [59] | 2019 | BC cells | CAFs | 3D Microfluidic Device | Demonstrate the ability of this co-culture model to recapitulate the TME |
Zhang et al. [66] | 2022 | MDA-MB-231 cells | CAFs, ECMs, HMFs | Organ-on-chip | Monitor patient primary tumors’ responses to anti-PD1 treatment |
Wallstabe, L. et al. [67] | 2019 | MDA-MB-231 | ECM | Microphysiologic 3D culture | ROR1-CAR T-cell therapy |
Thakur, A, et al. [68] | 2021 | Multiple BC cell lines | PBMC or MDSC | 3D tumorsphere model | Adoptive transfer of bispecific antibody armed activated T cells |
Ayuso et al. [69] | 2019 | MCF7 cells | ECM | 3D microfluidic device | NK cells immunotherapy |
Dees, S. et al. [70] | 2021 | TNBC cells | Human PBMCs | Adding cells to organoids | Combining T-cell-redirecting bispecific antibodies with ICIs |
Shelkey, E. et al. [71] | 2022 | 4T1 TNBC murine cells | Matched splenocytes | Adding cells to organoids | Investigate the factors affecting ICB response |
Zhou, Z. et al. [72] | 2021 | MDA-MB-468 cells | CAFs, CD8+ T cells | Adding cells to organoids | A high-throughput screen to identify epigenetic inhibitors |
Carter, M. E. et al. [73] | 2022 | BC tumor tissues | _ | _ | Investigate the effects of oncolytic virotherapy |
Behrens, M. D. et al. [74] | 2022 | MDA-MB-231, BC PDX | _ | _ | Investigate the effects of oncolytic virotherapy |