Fig. 4

HSDL2 knockdown upregulates SLC7A11 and suppresses ferroptosis in cholangiocarcinoma (CCA) cells. A Differential SLC7A11 mRNA expression between CCA tissues and adjacent tissues were determined using the GEPIA database. B Immunofluorescence was applied to analyze SLC7A11 expression in CCA tissues and adjacent tissues. C Correlation between HSDL2 expression and SLC7A11 expression in CCA datasets from the GEPIA database. D Western blotting of HSDL2 and SLC7A11 expression levels in CCA cells. E–G The relative reactive oxygen species (ROS) (E), malonaldehyde (MDA) (F), and GSH (G) levels in the indicated groups were measured using the corresponding kits. Data are presented as mean ± SD (n=3). *p < 0.05, **p < 0.01, and ***p < 0.001