Fig. 2

RNF213-SLC26A11 in addition to BCOR-CCNB3 fusion in the tumor in this study. A FISH analysis (with the Vysis EWSR1 (22q12) Dual Color) showed that no split signal of EWSR1 was detected (white arrows). B Schematic diagram (RNA sequencing and Sanger sequence of RT-PCR) showed a gene fusion connecting BCOR exon 15 to CCNB3 exon 5 in this tumor. C FISH study using BCOR break-apart probe demonstrated split of the red (5′) and green (3′) probes (arrows). D RNA sequencing and Sanger sequence of RT-PCR revealed a novel RNF213-SLC26A11 fusion connecting RNF213 exon 2 to SLC26A11 exon 8 in this BCOR-CCNB3 sarcoma