Fig. 8

RT-PCR analysis of NAB2-STAT6 fusion gene expression in a fresh-frozen specimen. Upper: PCR-amplified DNA fragments indicate a true fusion gene after sequencing. Lane M contains a 100-bp ladder marker. Lanes 1 and 2 show the products of PCR with the primer pair F-NAB2 ex6 forward-2/R-STAT6 exon 17 reverse, which yielded DNA fragments of approximately 219 bp. Lane NC shows the result of PCR using distilled water. Lower: Direct sequencing analysis indicated the fusion of NAB2 exon 6 and STAT6 exon 16