Fig. 5

Downregulation of TMPO induced the cell apoptosis of U251 cells. a, b Representative images showing Annexin V/7-ADD staining results in TMPO-shRNA- or TMPO-shRNA(S2)-infected U251 cells. c, d Statistical analysis of apoptosis in the U251 cells with TMPO knockdown. e The protein expression of cleaved PARP and cleaved caspase-3 in U251 cells was significantly increased after knockdown of the TMPO gene as verified by western blot. **p < 0.01, ***p < 0.001