Table 1 Primer sequences used for PCR
Location | Primer sequence | Size (in bp) |
|---|---|---|
c-KIT exon 9 | F: 5′-TCCTAGAGTAAGCCAGGGCTT-3′ | 283 |
R: 5′-TGGTAGACAGAGCCTAAACATCC-3′ | ||
c-KIT exon 11 | F: 5′-CCAGAGTGCTCTAATGACTG-3′ | 215 |
R: 5′-AGCCCCTGTTTCATACTGAC-3′ | ||
c-KIT exon 13 | F: 5′-GCTTGACATCAGTTTGCCAG-3′ | 193 |
R: 5′-AAAGGCAGCTTGGACACGGCTTTA-3′ | ||
c-KIT exon 17 | F: 5′-GGTTTTCTTTTCTCCTCCAACC-3′ | 203 |
R: 5′-GATTTACATTATGAAAGTCACAGG-3′ | ||
PDGFRA exon 12 | F: 5′-CCAGTTACCTGTCCTGGTCAT-3′ | 183 |
R: 5′-GGAGGTTACCCCATGGAACT-3′ | ||
PDGFRA exon 18 | F: 5′-TACAGATGGCTTGATCCTGAGT-3′ | 212 |
R: 5′-AGTGAAGGAGGATGAGCCTG-3′ | ||
BRAF exon 15 | F: 5′AAACTCTTCATAATGCTTGCTCTG 3′ | 200 |
R: 5′GGCCAAAAA TTTAATCAGTGG A 3′ |