Table 1 List of the various primary antibody markers
Antigen | Type | Dilution | Antigen retrieval | Source |
|---|---|---|---|---|
Vimentin | Monoclonal | 1:50 | Microwave | Dako, Produkionsveg, Glostrup, Denmark |
Cytokeratin (CK) | Monoclonal | 1:100 | Pronase (Enzymatic) | Dako |
CK 7 | Monoclonal | 1:50 | Microwave | Dako |
Epithelial membrane antigen (EMA) | Monoclonal | 1:100 | Pepsin (Enzymatic) | Dako |
Desmin | Monoclonal | 1:50 | Microwave | Dako |
CD34 | Monoclonal | 1:100 | Microwave | Dako |
Smooth muscle actin (SMA) | Monoclonal | 1:200 | Pepsin (Enzymatic) | Dako |
CK20 | Monoclonal | 1:100 | Microwave | Dako |
Carcinoembryonic antigen (CEA) | Polyclonal | 1:400 | Pepsin (Enzymatic) | Dako |
S-100 | Polyclonal | 1:300 | Pepsin (Enzymatic) | Dako |
Myo D-1 | Monoclonal | 1:20 | Pressure cooker and EDTA | Dako |
HMB-45 | Monoclonal | 1:50 | Microwave | Dako |
Leukocyte common antigen (LCA) | Monoclonal | 1:100 | Microwave | Dako |
C-KIT | Polyclonal | 1:100 | Microwave | Dako |
Calretinin | Monoclonal | 1:50 | Microwave | Dako |
CD31 | Monoclonal | 1:40 | Microwave | Dako |